However, in order to make use of all network card adapter features, you must install a proper LAN driver that enables the hardware. Recommended Anti-Rabbit secondary antibodies: Changing to another country might result in loss of shopping cart. Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment. Electrotransfer to nitrocellulose membrane Wash sections two times in dH 2 O for 5 min each. Mount sections with coverslips and mounting medium
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Allow cells to fix for 15 min at room temperature. Research studies have shown that NDRG1 may also play a role in cancer progression by promoting differentiation, inhibiting growth, and modulating metastasis and angiogenesis 3,4,6,8,9. Incubate 30 min on ice.
Dilute to 1X with dH 2 O. Wash sections in dH 2 O two times for 5 min each. Prepare solutions with reverse osmosis deionized RODI or equivalent grade water. NDRG1 is ubiquitously expressed and highly responsive to a variety of stress signals including DNA damage 4hypoxia 5and elevated levels of nickel and calcium 2.
Problems can arise when your hardware device is too old or not supported any longer.
Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. To prepare 10 ml, add 0.
Remove antibody solution and wash sections with wash buffer three times for 5 min each. Antigen Unmasking For Citrate: Aspirate media from cultures; wash cells with 1X PBS; aspirate.
CST – Phospho-NDRG1 (Thr) (D98G11) XP® Rabbit mAb
Wash by centrifugation in incubation buffer. Incubate for at least 5 min at room temperature. Incubate specimen in fluorochrome-conjugated secondary antibody diluted in Antibody Dilution Buffer for 1—2 hr at room temperature in the dark.
Do not allow px to dry at any time during this procedure. Would you like to visit your country specific website? To Purchase S View sizes. Rinse three times in 1X PBS for 5 min each.
Take into consideration that is not recommended to install the driver on Operating Systems other than stated ones.
Aspirate blocking solution, apply diluted primary antibody. If the driver is already installed on your system, updating overwrite-installing may fix various issues, add new functions, or just upgrade to the available version. Find answers on our FAQs page. Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature. Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available.
Incubate for 1 hr at room temperature. To install this package, you must follow the steps bellow: Aspirate fixative, rinse three times in 1X PBS for 5 min each. Volumes are for 10 cm x 10 cm cm 2 of membrane; for different sized membranes, adjust volumes accordingly.
Please refer to primary antibody datasheet or product xxp for recommended antibody dilution. Remember to perform a system reboot once done, to allow 8101 changes to take effect properly.
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Immerse slides in dH 2 O. Human, Mouse, Rat, Monkey. Electrotransfer to nitrocellulose membrane Fix for 15 min at room temperature.